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Titel:

A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarin.

Dokumenttyp:
Comparative Study; Journal Article
Autor(en):
Buters, J T; Schiller, C D; Chou, R C
Abstract:
The O-deethylation of 7-ethoxy-4-trifluoromethylcoumarin (EFC) by liver microsomes has been assessed as a method for monitoring the activity of cytochrome P450. The principle advantage of this substrate is the formation of a fluorescent product 7-hydroxy-4-trifluoromethylcoumarin (HFC) which can be assayed directly in the reaction medium. For rat microsomes the deethylated product was confirmed as the main metabolite, the reaction rate was linear with respect to both time and microsomal protein...     »
Zeitschriftentitel:
Biochem Pharmacol
Jahr:
1993
Band / Volume:
46
Heft / Issue:
9
Seitenangaben Beitrag:
1577-84
Volltext / DOI:
doi:10.1016/0006-2952(93)90326-r
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/8240414
Print-ISSN:
0006-2952
TUM Einrichtung:
Molekulare Allergologie (Prof. Schmidt-Weber)
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