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Title:

Characterization of retroviral vector derived DNA-isoforms by PCR and sequencing

Document type:
Article
Author(s):
Stellberger, Thorsten; Stockmar, Iris; Draxler, Johannes; Dhar, Prabir; Pavlovic, Melanie; Anton, Martina; Koehler, Nina; Dinkelmeier, Anna; Haase, Maren; Schick, Markus; Keller, Ulrich; Busch, Ulrich; Baiker, Armin
Abstract:
For the analytical surveillance of genetic engineering operations suitable methods for characterization of used (recombinant) cell lines and used transfer vector plasmids are needed. The purpose of the described method is the identification of retroviral DNA-isoforms based on lenti-or gammaretrovirus (HIV-1 or MLV). The primers, used in an optimized touch-down PCR protocol, specifically amplify transfer-vector encoded vector genomes and proviruses integrated into stably transduced vertebrate cel...     »
Journal title abbreviation:
J Verbrauch Lebensm
Year:
2019
Journal volume:
14
Journal issue:
2
Pages contribution:
157-165
Fulltext / DOI:
doi:10.1007/s00003-019-01215-7
Print-ISSN:
1661-5751
TUM Institution:
III. Medizinische Klinik und Poliklinik (Hämatologie / Onkologie); Institut für Experimentelle Onkologie und Therapieforschung
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