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Document type:
Journal Article; Research Support, Non-U.S. Gov't 
Author(s):
Romanelli, Elisa; Sorbara, Catherine D; Niki?, Ivana; Dagkalis, Athanasios; Misgeld, Thomas; Kerschensteiner, Martin 
Title:
Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes. 
Abstract:
Here we provide a protocol for rapidly labeling different cell types, distinct subcellular compartments and key injury mediators in the spinal cord of living mice. This method is based on the application of synthetic vital dyes to the surgically exposed spinal cord. Suitable vital dyes applied in appropriate concentrations lead to reliable in vivo labeling, which can be combined with genetic tags and in many cases preserved for postfixation analysis. In combination with in vivo imaging, this app...    »
 
Journal title abbreviation:
Nat Protoc 
Year:
2013 
Journal volume:
Journal issue:
Pages contribution:
481-90 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
1754-2189 
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)