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Titel:

The use of a laser for correlating light and electron microscopic images in thick tissue specimens.

Dokumenttyp:
Journal Article; Book Chapter; Review
Autor(en):
Bishop, Derron; Nikic, Ivana; Kerschensteiner, Martin; Misgeld, Thomas
Abstract:
Correlating images between light and electron microscopy is difficult especially in tissue specimens with a substantial z-dimension. To facilitate correlated light and electron microscopy (CLEM) in thick tissue specimens, we describe a basic method of using a femto-pulsed near-infrared laser to burn precise three-dimensional fiducial markers that circumscribe cells or regions of interest for easy identification between imaging methods. This rapid and reliable approach permits traditional fixatio...     »
Zeitschriftentitel:
Methods Cell Biol
Jahr:
2014
Band / Volume:
124
Seitenangaben Beitrag:
323-37
Sprache:
eng
Volltext / DOI:
doi:10.1016/B978-0-12-801075-4.00015-X
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/25287848
Print-ISSN:
0091-679X
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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