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- Original title:
- Charakterisierung thermostabiler Enzyme aus funktionell durchforsteten metagenomischen Genbanken
- Translated title:
- Characterization of thermostable enzymes obtained from functional screenings of metagenomic libraries
- Author:
- Pham, Vu Thuy Trang
- Year:
- 2018
- Document type:
- Dissertation
- Faculty/School:
- Fakultät Wissenschaftszentrum Weihenstephan
- Advisor:
- Liebl, Wolfgang (Prof. Dr.)
- Referee:
- Liebl, Wolfgang (Prof. Dr.); Vogel, Rudi F. (Prof. Dr.)
- Language:
- de
- Subject group:
- BIO Biowissenschaften
- Keywords:
- Metagenom, Glykosidhydrolase Familie, Funktionelles Screening, Thermostabile Enzyme, Thermus thermophilus
- Translated keywords:
- metagenome, glycoside hydrolase, functional screening, thermostable enzyme, Thermus thermophilus
- TUM classification:
- BIO 250d
- Abstract:
- In dieser Arbeit wurde an und mit Methoden der funktionellen Metagenomanalyse gearbeitet. Die Expression von Genen großer metagenomischer DNA-Fragmente wurde vergleichend in den Wirtsbakterien Escherichia coli und Thermus thermophilus gemessen. Die Charakterisierung einer thermostabilen endo-Glukanase, EngU, deren Gen durch funktionelle Durchforstung einer Metagenom-Genbibliothek gefunden wurde, ergab, dass es sich um den Prototyp einer neuen Glykosidhydrolase-Enzymfamilie (GH148) handelt.
- Translated abstract:
- In this thesis, methods for functional metagenome analysis were applied and improved. The expression of genes on large metagenomic DNA fragments was measured comparatively in two host bacteria, Escherichia coli and Thermus thermophilus. Characterization of a thermostable endo-glucanase, EngU, whose gene was identified by functional screening of a metagenomic gene library, revealed that it represents the prototype member of a new glycoside hydrolase family (GH148).
- WWW:
- https://mediatum.ub.tum.de/?id=1415767
- Date of submission:
- 18.12.2017
- Oral examination:
- 11.04.2018
- File size:
- 3449606 bytes
- Pages:
- 203
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20180411-1415767-1-2
- Last change:
- 24.04.2018
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