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Title:

Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants

Document type:
Zeitschriftenaufsatz
Author(s):
Kaveh-Baghbaderani Y, Blank-Shim SA, Koch T, Berensmeier S:
Abstract:
The need for fast and convenient protein purification is enormous, not only on the industrial scale for the production of therapeutics but also in life science research. Most protein purification strategies require multiple steps, which are time consuming, cost and resource intensive. Selective release of the intracellular target product can considerably facilitate downstream processing by minimizing the HCP concentration before subsequent purification processes. Here, we present a simple method...     »
Keywords:
Selective release Selective liberation One-step Freeze-thaw Protein purification Green fluorescent protein GFP
Journal title:
Protein Expression and Purification, Elsevier
Year:
2018
Covered by:
Web of Science
Language:
en
Fulltext / DOI:
doi:10.1016/j.pep.2018.07.014
WWW:
https://www.sciencedirect.com/science/article/pii/S1046592818303486
TUM Institution:
Professur für Selektive Trenntechnik
Ingested:
10.12.2018
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