Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy.

Gerisch, Marie; Smettan, Jan; Ebert, Sabine; Athelogou, Maria; Brand-Saberi, Beate; Spindler, Nick; Mueller, Wolf C; Giri, Shibashish; Bader, Augustinus

doi:10.3389/fgene.2018.00072

2018

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Title:: Qualitative and Quantitative Analysis of Cardiac Progenitor Cells in Cases of Myocarditis and Cardiomyopathy.
Document type:: Journal Article
Author(s):: Gerisch, Marie; Smettan, Jan; Ebert, Sabine; Athelogou, Maria; Brand-Saberi, Beate; Spindler, Nick; Mueller, Wolf C; Giri, Shibashish; Bader, Augustinus
Abstract:: We aimed to identify and quantify CD117 and CD90 endogenous cardiac progenitor cells (CPC) in human healthy and diseased hearts. We hypothesize that these cells perform a locally acting, contributing function in overcoming medical conditions of the heart by endogenous means. Human myocardium biopsies were obtained from 23 patients with the following diagnoses: Dilatative cardiomyopathy (DCM), ischemic cardiomyopathy (ICM), myocarditis, and controls from healthy cardiac patients. High-resolution scanning microscopy of the whole slide enabled a computer-based immunohistochemical quantification of CD117 and CD90. Those signals were evaluated by Definiens Tissue Phenomics® Technology. Co-localization of CD117 and CD90 was determined by analyzing comparable serial sections. CD117/CD90 cardiac cells were detected in all biopsies. The highest expression of CD90 was revealed in the myocarditis group. CD117 was significantly higher in all patient groups, compared to healthy specimens ( < 0.05). The highest co-expression was found in the myocarditis group (6.75 ± 3.25 CD90CD117 cells/mm) followed by ICM (4 ± 1.89 cells/mm), DCM (1.67 ± 0.58 cells/mm), and healthy specimens (1 ± 0.43 cells/mm). We conclude that the human heart comprises a fraction of local CD117 and CD90 cells. We hypothesize that these cells are part of local endogenous progenitor cells due to the co-expression of CD90 and CD117. With novel digital image analysis technologies, a quantification of the CD117 and CD90 signals is available. Our experiments reveal an increase of CD117 and CD90 in patients with myocarditis.
Journal title abbreviation:: Front Genet
Year:: 2018
Journal volume:: 9
Pages contribution:: 72
Language:: eng
Fulltext / DOI:: doi:10.3389/fgene.2018.00072
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/29559994
Print-ISSN:: 1664-8021
TUM Institution:: Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Plastische Chirurgie und Handchirurgie (Prof. Machens)Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)2018