Breakage of the fragile site FRA16D disrupts the (WW Domain Containing Oxidoreductase) tumor suppressor gene in osteosarcoma. However, the frequency of breakage is not sufficient to explain the rate of loss in pathogenesis. The involvement of non-coding RNA transcripts is proposed due to their accumulation at fragile sites, where they are advocated to influence specific chromosomal regions associated with malignancy. The long ncRNA (promoter of antisense radiation-induced circulating long non-coding RNA) is transiently elevated in response to irradiation and influences epigenetic silencing modification within . It now emerges that elevated levels are significantly associated with FRA16D non-breakage in OS patients. Although not associated with overall survival, high levels were found to be significantly linked to metastasis free outcome. The transcription of both and are transiently responsive to exposure to low doses of radiation in osteosarcoma cell lines. Herein, a relationship between and transcription is suggested in human osteosarcoma cell lines representing alternative genetic backgrounds. over-expression ameliorated promoter activity in U2OS harboring FRA16D non-breakage. It can be concluded that the lncRNA influences the tumor suppressor and in the absence of FRA16D breakage, it is associated with OS metastasis-free survival.     «

Breakage of the fragile site FRA16D disrupts the (WW Domain Containing Oxidoreductase) tumor suppressor gene in osteosarcoma. However, the frequency of breakage is not sufficient to explain the rate of loss in pathogenesis. The involvement of non-coding RNA transcripts is proposed due to their accumulation at fragile sites, where they are advocated to influence specific chromosomal regions associated with malignancy. The long ncRNA (promoter of antisense radiation-induced circulating long no...     »