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Titel:

Bacterial encapsulins as orthogonal compartments for mammalian cell engineering.

Dokumenttyp:
Article
Autor(en):
Sigmund, Felix; Massner, Christoph; Erdmann, Philipp; Stelzl, Anja; Rolbieski, Hannes; Desai, Mitul; Bricault, Sarah; Woerner, Tobias P.; Snijder, Joost; Geerlof, Arie; Fuchs, Helmut; de Angelis, Martin Hrabe; Heck, Albert J. R.; Jasanoff, Alan; Ntziachristos, Vasilis; Plitzko, Juergen; Westmeyer, Gil G.
Abstract:
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulatio...     »
Zeitschriftentitel:
Nat Commun
Jahr:
2018
Band / Volume:
9
Heft / Issue:
1
Volltext / DOI:
doi:10.1038/s41467-018-04227-3
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/29777103
Print-ISSN:
2041-1723
TUM Einrichtung:
Klinik und Poliklinik für Nuklearmedizin; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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