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Title:

High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy.

Document type:
Journal Article
Author(s):
Zhang, Ziyang; Slobodianski, Alex; Arnold, Astrid; Nehlsen, Jessica; Hopfner, Ursula; Schilling, Arndt F; Perisic, Tatjana; Machens, Hans-Günther
Abstract:
Dermal fibroblast is a powerful tool for the study ofDNA delivery in development of both cell therapy and tissue engineering products. Using genetic modification, fibroblasts can be diversely adapted and made suitable for clinical gene therapy. In this study, we first compared several non-viral transfection methods including nucleofection in rat and human primary dermal fibroblast. In addition, the original protocol for nucleofection of primary mammalian fibroblasts was modi...     »
Journal title abbreviation:
Int J Med Sci
Year:
2017
Journal volume:
14
Journal issue:
9
Pages contribution:
798-803
Language:
eng
Fulltext / DOI:
doi:10.7150/ijms.19241
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/28824316
TUM Institution:
Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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