Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts.

Perisic, Tatjana; Zhang, Ziyang; Foehr, Peter; Hopfner, Ursula; Klutz, Kathrin; Burgkart, Rainer H; Slobodianski, Alexei; Goeldner, Moritz; Machens, Hans-Günther; Schilling, Arndt F

doi:10.1371/journal.pone.0174860

journal article

Title:: Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts.
Document type:: Journal Article
Author(s):: Perisic, Tatjana; Zhang, Ziyang; Foehr, Peter; Hopfner, Ursula; Klutz, Kathrin; Burgkart, Rainer H; Slobodianski, Alexei; Goeldner, Moritz; Machens, Hans-Günther; Schilling, Arndt F
Abstract:: Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine.
Journal title abbreviation:: PLoS ONE
Year:: 2017
Journal volume:: 12
Journal issue:: 4
Pages contribution:: e0174860
Language:: eng
Fulltext / DOI:: doi:10.1371/journal.pone.0174860
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/28380080
Print-ISSN:: 1932-6203
TUM Institution:: Klinik und Poliklinik für Orthopädie und Sportorthopädie; Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Plastische Chirurgie und Handchirurgie (Prof. Machens)Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)2017

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Orthopädie und Sportorthopädie (Prof. von Eisenhart-Rothe)2017