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Titel:

Isolation of peripheral blood CD4(+) T cells using RosetteSep and MACS for studies of DNA turnover by deuterium labeling.

Dokumenttyp:
Journal Article; Article
Autor(en):
Busch, R; Cesar, D; Higuera-Alhino, D; Gee, T; Hellerstein, MK; McCune, JM
Abstract:
Tracking deuterium ((2)H) incorporation into cellular DNA, after administration of (2)H(2)O or (2)H(2)-glucose, is a recently developed, broadly applicable method for measuring in vivo cell proliferation and turnover that can be used safely in humans. This approach has been used to evaluate the turnover of T-cell subpopulations purified from the peripheral blood of HIV-1-infected patients using fluorescence-activated cell sorting (FACS). A requirement for widespread adoption of this approach for...     »
Zeitschriftentitel:
J Immunol Methods
Jahr:
2004
Band / Volume:
286
Heft / Issue:
1-2
Seitenangaben Beitrag:
97-109
Sprache:
eng
Volltext / DOI:
doi:10.1016/j.jim.2003.12.012
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/15087225
Print-ISSN:
0022-1759
TUM Einrichtung:
Institut für Medizinische Statistik und Epidemiologie
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