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Titel:

Differential analysis of Saccharomyces cerevisiae mitochondria by free flow electrophoresis.

Dokumenttyp:
Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Zischka, H; Braun, RJ; Marantidis, EP; Büringer, D; Bornhövd, C; Hauck, SM; Demmer, O; Gloeckner, CJ; Reichert, AS; Madeo, F; Ueffing, M
Abstract:
One major problem concerning the electrophoresis of mitochondria is the heterogeneity of mitochondrial appearance especially under pathological conditions. We show here the use of zone electrophoresis in a free flow electrophoresis device (ZE-FFE) as an analytical sensor to discriminate between different yeast mitochondrial populations. Impairment of the structural properties of the organelles by hyperosmotic stress resulted in broad separation profiles. Conversely untreated mitochondria gave ri...     »
Zeitschriftentitel:
Mol Cell Proteomics
Jahr:
2006
Band / Volume:
5
Heft / Issue:
11
Seitenangaben Beitrag:
2185-200
Sprache:
eng
Volltext / DOI:
doi:10.1074/mcp.T600018-MCP200
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/16917120
Print-ISSN:
1535-9476
TUM Einrichtung:
Institut für Humangenetik
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