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Titel:

Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresis.

Dokumenttyp:
Journal Article; Article
Autor(en):
Zischka, H; Weber, G; Weber, PJ; Posch, A; Braun, RJ; Bühringer, D; Schneider, U; Nissum, M; Meitinger, T; Ueffing, M; Eckerskorn, C
Abstract:
The analysis of complex cellular proteomes by means of two-dimensional gel electrophoresis (2-DE) is significantly limited by the power of resolution of this technique. Although subcellular fractionation can be a fundamental first step to increase resolution, it frequently leads to preparations contaminated with other cellular structures. Here, we chose mitochondria of Saccharomyces cerevisiae to demonstrate that an integrated zone-electrophoretic purification step (ZE), with a free-flow electro...     »
Zeitschriftentitel:
Proteomics
Jahr:
2003
Band / Volume:
3
Heft / Issue:
6
Seitenangaben Beitrag:
906-16
Sprache:
eng
Volltext / DOI:
doi:10.1002/pmic.200300376
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/12833514
Print-ISSN:
1615-9853
TUM Einrichtung:
Institut für Humangenetik
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