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2012
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Document type:
Journal Article
Author(s):
Herranz, F; Schmidt-Weber, CB; Shamji, MH; Narkus, A; Ruiz-Cabello, J; Vilar, R
Title:
Superparamagnetic iron oxide nanoparticles conjugated to a grass pollen allergen and an optical probe.
Abstract:
In this study we report the development of a bioconjugate between superparamagnetic iron oxide nanoparticles and Phl p5a (one of the major allergens from grass pollen). The bioconjugate also contains an optical probe (Alexa647) conjugated to the nanoparticle via biotin-streptavidin association. We show that this conjugate has a range of features that makes it a very promising candidate to image the localization of this allergen in vivo: (a) upon conjugation to the iron oxide nanoparticles, the allergen retains its ability to interact with IgE antibodies; (b) the magnetic properties of the iron oxide core of this bioconjugate are suitable for MR imaging; and (c) Alexa647 fluorophore retains its emission properties once attached to the iron oxide nanoparticles, yielding a dual modality MRI-optical probe. Copyright © 2012 John Wiley & Sons, Ltd.
Journal title abbreviation:
Contrast Media Mol Imaging
Year:
2012
Journal volume:
7
Journal issue:
4
Pages contribution:
435-9
Language:
eng
Fulltext / DOI:
doi:10.1002/cmmi.1466
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/22649050
Print-ISSN:
1555-4309
TUM Institution:
Molekulare Allergologie (Prof. Schmidt-Weber)
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2012
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