EGFR-specific T cell frequencies correlate with EGFR expression in head and neck squamous cell carcinoma.

Schuler, PJ; Boeckers, P; Engers, R; Boelke, E; Bas, M; Greve, J; Dumitru, CA; Lehnerdt, GF; Ferris, RL; Andrade Filho, PA; Brandau, S; Lang, S; Whiteside, TL; Hoffmann, TK

doi:10.1186/1479-5876-9-168

2011

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Title:: EGFR-specific T cell frequencies correlate with EGFR expression in head and neck squamous cell carcinoma.
Document type:: Journal Article
Author(s):: Schuler, PJ; Boeckers, P; Engers, R; Boelke, E; Bas, M; Greve, J; Dumitru, CA; Lehnerdt, GF; Ferris, RL; Andrade Filho, PA; Brandau, S; Lang, S; Whiteside, TL; Hoffmann, TK
Abstract:: In head and neck squamous cell carcinoma (HNSCC), expression levels of the epidermal growth factor receptor (EGFR) correlate with poor prognosis and decreased survival rates. As the mechanisms responsible for cellular immune response to EGFR in vivo remain unclear, the frequency and function of EGFR-specific cytotoxic T cells (CTL) was determined in HNSCC patients.The frequency of CTL specific for the HLA-A2.1-restricted EGFR-derived YLN peptide (YLNTVQPTCV) and KLF peptide (KLFGTSGQKT) was determined in 16 HLA-A2.1+ HNSCC patients and 16 healthy HLA-A2.1+ individuals (NC) by multicolor flow cytometry. Patients' results were correlated to EGFR expression obtained by immunohistochemistry in corresponding tumor sections. Proliferation and anti-tumor activity of peptide-specific CTL was demonstrated by in vitro stimulation with dendritic cells pulsed with the peptides.Frequency of EGFR-specific CTL correlated significantly with EGFR expression in tumor sections (p = 0.02, r2 = 0.6). Patients with elevated EGFR scores (> 7) had a significantly higher frequency of EGFR-specific CTL than NC and patients with low EGFR scores (< 7). EGFR-specific CTL from cancer patients were expanded ex vivo and produced IFN-? upon recognition of EGFR+ target cells.EGFR expressed on HNSCC cells induces a specific immune response in vivo. Strategies for expansion of EGFR-specific CTL may be important for future immunotherapy of HNSCC patients.
Journal title abbreviation:: J Transl Med
Year:: 2011
Journal volume:: 9
Pages contribution:: 168
Language:: eng
Fulltext / DOI:: doi:10.1186/1479-5876-9-168
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/21970318
Print-ISSN:: 1479-5876
TUM Institution:: Hals-Nasen-Ohrenklinik und Poliklinik
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde (Prof. Wollenberg)2011