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Titel:

Live fluorescent RNA-based detection of pluripotency gene expression in embryonic and induced pluripotent stem cells of different species.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Lahm, Harald; Doppler, Stefanie; Dreßen, Martina; Werner, Astrid; Adamczyk, Klaudia; Schrambke, Dominic; Brade, Thomas; Laugwitz, Karl-Ludwig; Deutsch, Marcus-André; Schiemann, Matthias; Lange, Rüdiger; Moretti, Alessandra; Krane, Markus
Abstract:
The generation of induced pluripotent stem (iPS) cells has successfully been achieved in many species. However, the identification of truly reprogrammed iPS cells still remains laborious and the detection of pluripotency markers requires fixation of cells in most cases. Here, we report an approach with nanoparticles carrying Cy3-labeled sense oligonucleotide reporter strands coupled to gold-particles. These molecules are directly added to cultured cells without any manipulation and gene expressi...     »
Zeitschriftentitel:
Stem Cells
Jahr:
2015
Band / Volume:
33
Heft / Issue:
2
Seitenangaben Beitrag:
392-402
Sprache:
eng
Volltext / DOI:
doi:10.1002/stem.1872
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/25335772
Print-ISSN:
1066-5099
TUM Einrichtung:
Arbeitsgruppe Tissue Engineering und Regenerative Medizin; I. Medizinische Klinik und Poliklinik (Kardiologie); Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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