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Titel:

Protocol for whole-mount immunofluorescence staining of ECM gel-embedded innervated pancreatic organoids.

Dokumenttyp:
Journal Article
Autor(en):
Beşikcioğlu, Hüseyin Erdinç; Yurteri, Ümmügülsüm; Ye, Linhan; Zhang, Fang; Moretti, Alessandra; Gürcinar, Ibrahim Halil; Dogruöz, Alper; Karakas, Didem; Friess, Helmut; Ceyhan, Guralp Onur; Istvanffy, Rouzanna; Ekin Demir, Ihsan
Abstract:
The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibo...     »
Zeitschriftentitel:
STAR Protoc
Jahr:
2024
Band / Volume:
5
Heft / Issue:
2
Volltext / DOI:
doi:10.1016/j.xpro.2024.103132
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/38875112
TUM Einrichtung:
Professur für Regenerative Medizin bei Kardiovaskulären Erkrankungen (Prof. Moretti)
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