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Titel:

High-throughput optical action potential recordings in hiPSC-derived cardiomyocytes with a genetically encoded voltage indicator in the AAVS1 locus.

Dokumenttyp:
Article; Journal Article
Autor(en):
Zhang, Fangfang; Meier, Anna B; Poch, Christine M; Tian, Qinghai; Engelhardt, Stefan; Sinnecker, Daniel; Lipp, Peter; Laugwitz, Karl-Ludwig; Moretti, Alessandra; Dorn, Tatjana
Abstract:
Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) represent an excellent in vitro model in cardiovascular research. Changes in their action potential (AP) dynamics convey information that is essential for disease modeling, drug screening and toxicity evaluation. High-throughput optical AP recordings utilizing intramolecular Förster resonance energy transfer (FRET) of the voltage-sensitive fluorescent protein (VSFP) have emerged as a substitute or complement to the r...     »
Zeitschriftentitel:
Front Cell Dev Biol
Jahr:
2022
Band / Volume:
10
Volltext / DOI:
doi:10.3389/fcell.2022.1038867
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/36274846
TUM Einrichtung:
Institut für Pharmakologie und Toxikologie; Klinik und Poliklinik für Innere Medizin I, Kardiologie; Professur für Regenerative Medizin bei Kardiovaskulären Erkrankungen (Prof. Moretti)
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