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Titel:

Optimizing of the basophil activation test: Comparison of different basophil identification markers.

Dokumenttyp:
journal article
Autor(en):
Eberlein, Bernadette; Hann, Rebekka; Eyerich, Stefanie; Pennino, Davide; Ring, Johannes; Schmidt-Weber, Carsten B; Buters, Jeroen
Abstract:
Background Flowcytometric identification of basophils is a prerequisite for measuring activation of basophils with IgE-dependent or IgE-independent stimuli. Aim of this study was to compare different marker combinations in a simultaneous multicolor flowcytometric measurement. Methods Ten patients with a grass pollen allergy and 3 controls were included in the study. Basophilic cells were gated by using anti-CCR3647, anti-IgE, anti-CRTH2, anti-CD203c und anti-CD3. Cells were activated by a monocl...     »
Zeitschriftentitel:
Cytometry B Clin Cytom
Jahr:
2014
Sprache:
eng
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/25382789
Print-ISSN:
1552-4949
TUM Einrichtung:
Institut für Molekulare Allergologie und Umweltforschung
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