Expression of the Tumor-associated Mucin 1 Epitope Analyzed with the Humanized PankoMab-GEX(TM) Antibody in Malignant and Normal Tissues of the Head and Neck.

Wiest, Irmi; Alexiou, Christoph; Friese, Klaus; Betz, Peter; Tübel, Jutta; Goletz, Steffen; Dian, Darius; Jeschke, Udo

2016

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Dokumenttyp:: journal article
Autor(en):: Wiest, Irmi; Alexiou, Christoph; Friese, Klaus; Betz, Peter; Tübel, Jutta; Goletz, Steffen; Dian, Darius; Jeschke, Udo
Titel:: Expression of the Tumor-associated Mucin 1 Epitope Analyzed with the Humanized PankoMab-GEX(TM) Antibody in Malignant and Normal Tissues of the Head and Neck.
Abstract:: During neoplasia, glycosylation changes. In this setting, mucins, especially mucin 1 (MUC1), become carriers for oncofetal carbohydrates and relieve invasive growth. The recently described tumor-associated MUC1 epitope TA-MUC1 is primarily restricted to malignancies and is overexpressed in these tissues. The humanized monoclonal antibody PankoMab-GEX specifically recognizes TA-MUC1.Laryngeal cancer specimens (n=125) and normal tissue of head and neck (n=7) were used in this study. Paraffin-embedded sections were incubated with PankoMab-GEX. Staining reaction was carried out using peroxidase (POD) labeling and diaminobenzidine (DAB). Breast cancer tissue was used as positive control and negative control used non-specific mouse IgM. Semi-quantitative evaluation by two independent double-blinded investigators, including a pathologist, used the immunoreactive score (IRS) of Remmele and Stegner.A total of 31 out of 125 laryngeal cancer specimens were classified as G1. Of these, 22 (71%) were completely negative for TA-MUC1, the remaining 9 showed very weak staining, with an IRS of 2. A total of 94 cases of cancer specimens were classified as G2 and G3; 34 of them were also negative, but 60 had an IRS of up to 9. All investigated normal tissue of the upper aerodigestive tract was completely negative for TA-MUC1.G1 tumors are completely negative or do not reach an IRS relevant range. The finding that G1 tumors are completely negative for TA-MUC1 or have IRS<=2 can be helpful for histopathological examination, especially concerning tumor grading. Therefore, this antibody holds great potential for use as a therapeutic antibody in laryngeal cancer.
Zeitschriftentitel:: Anticancer Res
Jahr:: 2016
Band / Volume:: 36
Heft / Issue:: 6
Seitenangaben Beitrag:: 3179-84
Sprache:: eng
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/27272845
Print-ISSN:: 0250-7005
TUM Einrichtung:: Orthopädische und Sportorthopädische Klinik und Poliklinik
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Unfallchirurgie (Prof. Biberthaler)2016