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Title:

Magnetic nanoparticle and magnetic field assisted siRNA delivery in vitro.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Mykhaylyk, Olga; Sanchez-Antequera, Yolanda; Vlaskou, Dialechti; Cerda, Maria Belen; Bokharaei, Mehrdad; Hammerschmid, Edelburga; Anton, Martina; Plank, Christian
Abstract:
This chapter describes how to design and conduct experiments to deliver siRNA to adherent cell cultures in vitro by magnetic force-assisted transfection using self-assembled complexes of small interfering RNA (siRNA) and cationic lipids or polymers that are associated with magnetic nanoparticles (MNPs). These magnetic complexes are targeted to the cell surface by the application of a gradient magnetic field. A further development of the magnetic drug-targeting concept is combining it with an ult...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2015
Journal volume:
1218
Pages contribution:
53-106
Language:
eng
Fulltext / DOI:
doi:10.1007/978-1-4939-1538-5_5
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/25319646
Print-ISSN:
1064-3745
TUM Institution:
Institut für Experimentelle Onkologie und Therapieforschung
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