Comparison of two assays for molecular determination of rifampin resistance in clinical samples from patients with Buruli ulcer disease.

Titel:: Comparison of two assays for molecular determination of rifampin resistance in clinical samples from patients with Buruli ulcer disease.
Dokumenttyp:: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):: Jansson, Moritz; Beissner, Marcus; Phillips, Richard Odame; Badziklou, Kossi; Piten, Ebekalisai; Maman, Issaka; Sarfo, Fred Stephen; Huber, Kristina Lydia; Rhomberg, Agata; Symank, Dominik; Wagner, Magdalena; Wiedemann, Franz; Nitschke, Jörg; Banla Kere, Abiba; Herbinger, Karl-Heinz; Adjei, Ohene; Löscher, Thomas; Bretzel, Gisela
Abstract:: This study evaluates a novel assay for detecting rifampin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% M. ulcerans specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence.
Zeitschriftentitel:: J Clin Microbiol
Jahr:: 2014
Band / Volume:: 52
Heft / Issue:: 4
Seitenangaben Beitrag:: 1246-9
Sprache:: eng
Volltext / DOI:: doi:10.1128/JCM.03119-13
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/24478404
Print-ISSN:: 0095-1137
TUM Einrichtung:: Klinik für Anästhesiologie (DHM)
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