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Titel:

Fabrication and Characterization of Biphasic Silk Fibroin Scaffolds for Tendon/Ligament-to-Bone Tissue Engineering.

Dokumenttyp:
Journal Article; Article
Autor(en):
Font Tellado, Sonia; Bonani, Walter; Balmayor, Elizabeth R; Foehr, Peter; Motta, Antonella; Migliaresi, Claudio; Van Griensven, Martijn
Abstract:
Tissue engineering is an attractive strategy for tendon/ligament-to-bone interface repair. The structure and extracellular matrix composition of the interface are complex and allow for a gradual mechanical stress transfer between tendons/ligaments and bone. Thus, scaffolds mimicking the structural features of the native interface may be able to better support functional tissue regeneration. In this study, we fabricated biphasic silk fibroin scaffolds designed to mimic the gradient in collagen molecule alignment present at the interface. The scaffolds had two different pore alignments: anisotropic at the tendon/ligament side and isotropic at the bone side. Total porosity ranged from 50% to 80% and the majority of pores (80-90%) were <100-300 ?m. Young's modulus varied from 689 to 1322 kPa depending on the type of construct. In addition, human adipose-derived mesenchymal stem cells were cultured on the scaffolds to evaluate the effect of pore morphology on cell proliferation and gene expression. Biphasic scaffolds supported cell attachment and influenced cytoskeleton organization depending on pore alignment. In addition, the gene expression of tendon/ligament, enthesis, and cartilage markers significantly changed depending on pore alignment in each region of the scaffolds. In conclusion, the biphasic scaffolds fabricated in this study show promising features for tendon/ligament-to-bone tissue engineering.
Zeitschriftentitel:
Tissue Eng Part A
Jahr:
2017
Band / Volume:
23
Heft / Issue:
15-16
Seitenangaben Beitrag:
859-872
Sprache:
eng
Volltext / DOI:
doi:10.1089/ten.TEA.2016.0460
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/28330431
Print-ISSN:
1937-3341
TUM Einrichtung:
Klinik und Poliklinik für Orthopädie und Sportorthopädie; Klinik und Poliklinik für Unfallchirurgie
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