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Title:

Expression and Cellular Localization of CXCR4 and CXCL12 in Human Carotid Atherosclerotic Plaques.

Document type:
Journal Article; Article
Author(s):
Merckelbach, Sophie; van der Vorst, Emiel P C; Kallmayer, Michael; Rischpler, Christoph; Burgkart, Rainer; Döring, Yvonne; de Borst, Gert-Jan; Schwaiger, Markus; Eckstein, Hans-Henning; Weber, Christian; Pelisek, Jaroslav
Abstract:
The CXCR4/CXCL12 complex has already been associated with progression of atherosclerosis; however, its exact role is yet unknown. The aim of this study was to analyse the expression and cellular localization of CXCL12 and its receptor CXCR4 in human carotid atherosclerotic plaques. Carotid plaques ( = 58; 31 stable, 27 unstable, based on histological characterization of plaque morphology) were obtained during carotid endarterectomy, and 10 healthy vessels were used as a control. Expression of,,,andwas analysed at mRNA, and level expression of CXCR4, CXCR7 and CXCL12 was analysed at protein level. Cellular localization was determined using consecutive and double immunohistochemical (IHC) staining and microdissection. At mRNA level,andwere significantly higher expressed in stable carotid plaques compared with controls ( = 0.011, < 0.001 and < 0.001).mRNA expression was successively augmented toward unstable plaques ( < 0.001). At protein level, CXCR4, CXCR7 and CXCL12 expression was significantly increased in both stable ( = 0.001, < 0.001 and = 0.035, respectively) and unstable ( = 0.003, < 0.001 and = 0.045, respectively) plaques compared with controls. Using IHC, CXCR4 was particularly localized in macrophages and small neovessels. Microdissection confirmed strongest expression ofin macrophages within atherosclerotic plaques. Leukocytes and smooth muscle cells showedexpression as well. For, only microdissected areas with macrophages were positive. Expression of CXCR4 and CXCL12 was significantly increased in both stable and unstable carotid atherosclerotic plaques compared with healthy vessels, both at mRNA and protein level. CXCR4 and CXCL12 were localized particularly in macrophages.
Journal title abbreviation:
Thromb Haemost
Year:
2018
Journal volume:
118
Journal issue:
1
Pages contribution:
195-206
Language:
eng
Fulltext / DOI:
doi:10.1160/TH17-04-0271
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/29304539
Print-ISSN:
0340-6245
TUM Institution:
Fachgebiet Gefäßchirurgie (Prof. Eckstein); Klinik und Poliklinik für Nuklearmedizin; Klinik und Poliklinik für Orthopädie und Sportorthopädie
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