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Title:

Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.

Document type:
Zeitschriftenaufsatz
Author(s):
Mishra, Kanuj; Fuenzalida-Werner, Juan Pablo; Pennacchietti, Francesca; Janowski, Robert; Chmyrov, Andriy; Huang, Yuanhui; Zakian, Christian; Klemm, Uwe; Testa, Ilaria; Niessing, Dierk; Ntziachristos, Vasilis; Stiel, Andre C
Abstract:
Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they have yet to be used as sensors that measure the distribution of specific analytes at the nanoscale or in the tissues of live animals. Here we constructed the prototype of a photo-switchable Ca2+ sensor based on GCaMP5G that can be switched with 405/488-nm light and describe its molecular mechanisms at the structural level, including the importance...     »
Journal title:
Nature Biotechnology
Journal title abbreviation:
Nat Biotechnol
Year:
2022
Journal volume:
40
Journal issue:
4
Pages contribution:
598-605
Fulltext / DOI:
doi:10.1038/s41587-021-01100-5
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/34845372
WWW:
https://www.nature.com/articles/s41587-021-01100-5
Print-ISSN:
1087-0156
TUM Institution:
Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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