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Title:

Measuring T cell receptor and T cell gene expression diversity in antigen-responsive human CD4+ T cells.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Eugster, Anne; Lindner, Annett; Heninger, Anne-Kristin; Wilhelm, Carmen; Dietz, Sevina; Catani, Mara; Ziegler, Anette-G; Bonifacio, Ezio
Abstract:
T cells have diversity in TCR, epitope recognition, and cytokine production, and can be used for immune monitoring. Furthermore, clonal expansion of TCR families in disease may provide opportunities for TCR-directed therapies. We developed methodology for sequencing expressed genes of TCR alpha and beta chains from single cells and applied this to vaccine (tetanus-toxoid)-responsive CD4(+) T cells. TCR alpha and beta chains were both successfully sequenced in 1309 (43%) of 3038 CD4(+) T cells yi...     »
Journal title abbreviation:
J Immunol Methods
Year:
2013
Journal volume:
400-401
Pages contribution:
13-22
Language:
eng
Fulltext / DOI:
doi:10.1016/j.jim.2013.11.003
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/24239865
Print-ISSN:
0022-1759
TUM Institution:
Klinik und Poliklinik für Kinderheilkunde und Jugendmedizin
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