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- Title:
Effect of bone morphogenetic protein-2 on tendon-bone integration in an in vitro cell culture.
- Document type:
- Journal Article
- Author(s):
- Struewer, Johannes; Crönlein, Moritz; Ziring, Ewgeni; Schwarting, Tim; Kratz, Marita; Ruchholtz, Steffen; Frangen, Thomas Manfred
- Abstract:
- The goal of this study was to evaluate the influence of bone morphogenetic protein-2 (BMP-2) on tendon-bone integration in a bovine in vitro cell culture. Seventy-two bovine tendons were cultivated over 3 months. The effects of BMP-2 were evaluated by generation in 4 subgroups. The groups differed in 2 parameters: the application of BMP-2 and the application of primary bovine osteoblasts. Results were analyzed biochemically by determining alkaline phosphatase activity and histologic tendon calcification, both markers for graft incorporation. Histological analysis demonstrated a positive effect of BMP-2 on the production of extracellular matrix and therefore the induction of osteogenesis. In addition, the results showed a superior cell ingrowth on the tendon in the BMP-2-stimulated groups. Calcium carbonate-like structures and organized ossification zones could only be detected in the BMP-2-stimulated tendons. The histological results matched those of the biochemical alkaline phosphatase analysis. The highest alkaline phosphatase activity was detected using BMP-2 stimulation in the first month (P<.001). High alkaline phosphatase values suggest high osteoblast activity and a high potential for mineralization. Furthermore, a positive effect of BMP-2 on fibroblasts existed with regard to the overall integration process. These results confirm the positive influence and triggering effect of BMP-2 on the mineralization process. Bone morphogenetic protein-2 seems to accelerate and optimize tendon-bone integration in the early process of graft incorporation. Besides the influence of BMP-2 on bovine osteoblasts, an additional positive effect of BMP-2 on bovine fibroblasts was detected; therefore, graft incorporation may be carried out by osteoblasts and fibroblasts.
- Journal title abbreviation:
- Orthopedics
- Year:
- 2013
- Journal volume:
- 36
- Journal issue:
- 2
- Pages contribution:
- e200-6
- Fulltext / DOI:
- doi:10.3928/01477447-20130122-23
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/23380015
- Print-ISSN:
- 0147-7447
- TUM Institution:
- Klinik und Poliklinik für Unfallchirurgie
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