The Use of Human Sweat Gland-Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration.

Danner, S; Kremer, M; Petschnik, AE; Nagel, S; Zhang, Z; Hopfner, U; Reckhenrich, AK; Weber, C; Schenck, TL; Becker, T; Kruse, C; Machens, HG; Egana, JT

doi:10.1038/jid.2012.31

2012

Back
Back to start of result list
Permanent link for displayed object

Title:: The Use of Human Sweat Gland-Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration.
Document type:: Journal Article
Author(s):: Danner, S; Kremer, M; Petschnik, AE; Nagel, S; Zhang, Z; Hopfner, U; Reckhenrich, AK; Weber, C; Schenck, TL; Becker, T; Kruse, C; Machens, HG; Egana, JT
Abstract:: Vascularization is a key process in tissue engineering and regeneration and represents one of the most important issues in the field of regenerative medicine. Thus, several strategies to improve vascularization are currently under clinical evaluation. In this study, stem cells derived from human sweat glands were isolated, characterized, seeded in collagen scaffolds, and engrafted in a mouse full skin defect model for dermal regeneration. Results showed that these cells exhibit high proliferation rates and express stem cell and differentiation markers. Moreover, cells responded to angiogenic environments by increasing their migration (P<0.001) and proliferation (P<0.05) capacity and forming capillary-like structures. After seeding in the scaffolds, cells distributed homogeneously, interacting directly with the scaffold, and released bioactive molecules involved in angiogenesis, immune response, and tissue remodeling. In vivo, scaffolds containing cells were used to induce dermal regeneration. Here we have found that the presence of the cells significantly improved vascularization (P<0.001). As autologous sweat gland-derived stem cells are easy to obtain, exhibit a good proliferation capacity, and improve vascularization during dermal regeneration, we suggest that the combined use of sweat gland-derived stem cells and scaffolds for dermal regeneration might improve dermal regeneration in future clinical settings.
Journal title abbreviation:: J Invest Dermatol
Year:: 2012
Journal volume:: 132
Journal issue:: 6
Pages contribution:: 1707-16
Language:: eng
Fulltext / DOI:: doi:10.1038/jid.2012.31
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/22377762
Print-ISSN:: 0022-202X
TUM Institution:: Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)
BibTeX

Occurrences:

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Plastische Chirurgie und Handchirurgie (Prof. Machens)Lehrstuhl für Plastische Chirurgie und Handchirurgie (Prof. Machens)2012