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Title:

A novel expression and purification system for the production of enzymatic and biologically active human granzyme B.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Gehrmann, M; Doss, BT; Wagner, M; Zettlitz, KA; Kontermann, RE; Foulds, G; Pockley, AG; Multhoff, G
Abstract:
The serine protease granzyme B (grB) has previously been shown to induce perforin-independent apoptosis in membrane Hsp70 positive tumor cells in a number of in vitro experimental systems. Ongoing studies that are investigating the in vivo relevance of these findings by assessing the therapeutic potential of grB in a human xenograft tumor mouse model required the development of an expression system for the production of high yields of enzymatic and biologically active human grB. In order to main...     »
Journal title abbreviation:
J Immunol Methods
Year:
2011
Journal volume:
371
Journal issue:
1-2
Pages contribution:
8-17
Language:
eng
Fulltext / DOI:
doi:10.1016/j.jim.2011.06.007
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/21723287
Print-ISSN:
0022-1759
TUM Institution:
Klinik und Poliklinik für RadioOnkologie und Strahlentherapie
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