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- Document type:
- Zeitschriftenaufsatz
- Author(s):
- Schumann, S.; Stahl, C.A.; Möller, K.; Schneider, M.; Metzke, R.; Wall, W.A.; Priebe, H.-J.; Guttmann, J.
- Title:
- Contact-free determination of material characteristics using a newly developed pressure-operated strain-applying bioreactor
- Abstract:
- Experimental mechano-stimulation of biologic samples serves to understand fundamental processes in mechano biology. Unfortunately, present techniques do not allow contact-free investigation of material characteristics . We developed a mechano-stimulator that functions under the assumption that the counterforce generated by a biologic sample placed on a carrier membrane is extractable from the total signal. As material characteristics of biologic samples are poorly defined, we substituted them by polyurethane and latex membranes. We hypothesized that knowing the carrier membranes' compliance allows calculation of the sample's compliance. Compliances and mean errors of compliance measurements of latex membranes were larger than those of polyurethane ones (p<0.05). The differences between calculated and directly determined compliances were 3.9±2.7% and 6.7±4.8% (mean±SD) under static and dynamic mechano-stimulation, respectively (p>0.05). The variability of compliance values was serveral fold larger (p<0.05) when latex membranes served as test and polyurethane membranes as carrier membranes. There was high correlation between Young's modulus as determined from static compliance estimation and by uniaxial stretching in a tension-testing machine (r2=0.981, p=0.001). In conclusion, our mechano-stimulator allowed reliable contact-free determination of material charcteristics and may thus be suitable for contact-free assessment of biologic samples. Highly pliable carrier membranes should be used whenever very pliant material is investigated.
- Keywords:
- mechano-stimulator, mechanical characteristics, compliance, stretching experiments, Young's modulus
- Dewey Decimal Classification:
- 620 Ingenieurwissenschaften
- Journal title:
- Journal of Biomedical Materials Research: Part B
- Year:
- 2008
- Journal volume:
- 86B
- Journal issue:
- 2
- Pages contribution:
- 483-492
- Reviewed:
- ja
- Language:
- en
- Fulltext / DOI:
- doi:10.1002/jbm.b.31045
- Status:
- Verlagsversion / published
- Semester:
- WS 09-10
- Format:
- Text
BibTeX