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Titel:

Tracking stem cell differentiation in the setting of automated optogenetic stimulation.

Dokumenttyp:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
Autor(en):
Stroh, A; Tsai, HC; Wang, LP; Zhang, F; Kressel, J; Aravanis, A; Santhanam, N; Deisseroth, K; Konnerth, A; Schneider, MB
Abstract:
Membrane depolarization has been shown to play an important role in the neural differentiation of stem cells and in the survival and function of mature neurons. Here, we introduce a microbial opsin into ESCs and develop optogenetic technology for stem cell engineering applications, with an automated system for noninvasive modulation of ESC differentiation employing fast optogenetic control of ion flux. Mouse ESCs were stably transduced with channelrhodopsin-2 (ChR2)-yellow fluorescent protein an...     »
Zeitschriftentitel:
Stem Cells
Jahr:
2011
Band / Volume:
29
Heft / Issue:
1
Seitenangaben Beitrag:
78-88
Sprache:
eng
Volltext / DOI:
doi:10.1002/stem.558
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/21280159
Print-ISSN:
1066-5099
TUM Einrichtung:
Fachgebiet Neuroradiologie (Prof. Zimmer); Institut für Neurowissenschaften
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