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- Title:
A simplified method to assess affinity of insulin autoantibodies.
- Document type:
- Journal Article; Research Support, Non-U.S. Gov't; Article
- Author(s):
- Achenbach, P; Guo, LH; Gick, C; Adler, K; Krause, S; Bonifacio, E; Colman, PG; Ziegler, AG
- Abstract:
- Insulin autoantibodies (IAA) precede type 1 diabetes, but not all IAA-positive children develop other islet autoantibodies and disease. Diabetes risk can be stratified by laborious IAA affinity measurement using competition with multiple ligand concentrations. Here, we identify a single competitor concentration that discriminates low- and high-affinity IAA. Discrimination was achieved among 122 IAA-positive sera using 7.0 nM competitor which is 54-fold that of the assay radioligand concentration. Relative-binding <60% at this competitor concentration identified all 85 sera with affinities >=1.0×10? L/mol and none with lower affinities (P<0.0001), and 45 (96%) of 47 multiple islet autoantibody-positive sera (P<0.0001). IAA competition was further tested in a second set of 119 IAA-positive sera. Of these, 99 fulfilled high-affinity competition criteria of <60% relative-binding at 7.0 nM competitor including 89 (94%) of 95 sera with multiple islet autoantibodies (P<0.0001). Thus, increased IAA specificity can be achieved with simple modification to existing assays.
- Journal title abbreviation:
- Clin Immunol
- Year:
- 2010
- Journal volume:
- 137
- Journal issue:
- 3
- Pages contribution:
- 415-21
- Language:
- eng
- Fulltext / DOI:
- doi:10.1016/j.clim.2010.09.002
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/20920845
- Print-ISSN:
- 1521-6616
- TUM Institution:
- Klinik und Poliklinik für Kinderheilkunde und Jugendmedizin
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