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- Title:
Live or Let Die: Epithelial Flap Vitality and Keratocyte Proliferation Following LASEK and Epi-LASIK in Human Donor and Porcine Eyes.
- Document type:
- Journal Article
- Author(s):
- Angunawela, RI; Winkler von Mohrenfels, C; Kumar, A; O'Brart, DP; MARSHALL, J
- Abstract:
- To determine the relationship between epithelial flap vitality and stromal keratocyte proliferation following two epithelial refractive techniques: epi-LASIK and laser epithelial keratomileusis (LASEK).Human corneas were maintained in organ culture and underwent standard -6.00-diopter ablation. Rates of stromal keratocyte proliferation were detected 1 week postoperative using a Ki67 antibody specific to proliferating cells. Images were captured with a laser scanning confocal microscope and analyzed by a masked observer. Epithelial flap vitality was determined with propidium iodide using fresh porcine corneas. Epithelial flaps were created with Gebauer Epikeratome epi-LASIK or alcohol-assisted LASEK method. Flaps treated with 100% alcohol and uninjured corneas were used as controls.The number of proliferating keratocytes was greatest at 1 week in the epi-LASIK corneas (P<.001). Cell vitality was greatest in the epi-LASIK flaps and declined in the LASEK and 100% alcohol flaps (P<.001).In this in vitro setting, epi-LASIK results in an epithelial flap with significantly more live cells. There is also a greater number of proliferating stromal cells following epi-LASIK at 1 week. Based on these in vitro observations, epi-LASIK may result in greater levels of haze compared to LASEK.
- Journal title abbreviation:
- J Refract Surg
- Year:
- 2011
- Journal volume:
- 27
- Journal issue:
- 2
- Pages contribution:
- 111-8
- Language:
- eng
- Fulltext / DOI:
- doi:10.3928/1081597X-20100330-01
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/20415285
- Print-ISSN:
- 1081-597X
- TUM Institution:
- Klinik und Poliklinik für Augenheilkunde
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