Deficient Sekeletal Myogenesis after Genomic Aldehyde Dehydrogenase 1 Knockout:: Isoform ALDH1A1 and ALDH1A3 Proteins are Essential for Myotube Formation

Rihani, Laura Sophie

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Laura Sophie Rihani

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Original title:: Deficient Sekeletal Myogenesis after Genomic Aldehyde Dehydrogenase 1 Knockout:
Original subtitle:: Isoform ALDH1A1 and ALDH1A3 Proteins are Essential for Myotube Formation
Translated title:: Gestörte Myogenese in der Skelettmuskulatur nach Genomischen Aldehyd Dehydrogenase 1 Knockout:
Translated subtitle:: Isoformen ALDH1A1 und ALDH1A3 sind Essenziell für die Ausbidlung von Muskelfasern
Author:: Rihani, Laura Sophie
Year:: 2020
Document type:: Dissertation
Faculty/School:: Fakultät für Medizin
Advisor:: Schlegel, Jürgen (Prof. Dr.)
Referee:: Schlegel, Jürgen (Prof. Dr.); Multhoff, Gabriele (Prof. Dr.)
Language:: en
Subject group:: MED Medizin
Keywords:: Satellite Cells, Myogenesis, Differentiation, ALDH1, Isoforms 1A1 and 1A3
Translated keywords:: Satellitenzellen, Myogenese, Differenzierung, ALDH1, Isoformen 1A1 und 1A3
TUM classification:: MED 310d; MED 600d
Abstract:: Enzyme Aldehyde Dehydrogenase 1 isoforms 1A1 and 1A3 co-localize in human stallite cells, the stem cell population of skeletal muscle. The molecular function of 1A1 and 1A3 in myogenesis remains indistict. This study shows, that isoforms 1A1 and 1A3 proteins are essential in myogenic differentiation, since 1A1 and 1A3 knockout cells lost the potential to differentiate. Recombinant re-expression of isoforms in knockout cells recovered differentiation. Chemical inhibition of enzymatic activity leads to isoform protein accumulation and consequent differentiation. «
Enzyme Aldehyde Dehydrogenase 1 isoforms 1A1 and 1A3 co-localize in human stallite cells, the stem cell population of skeletal muscle. The molecular function of 1A1 and 1A3 in myogenesis remains indistict. This study shows, that isoforms 1A1 and 1A3 proteins are essential in myogenic differentiation, since 1A1 and 1A3 knockout cells lost the potential to differentiate. Recombinant re-expression of isoforms in knockout cells recovered differentiation. Chemical inhibition of enzymatic activity lea... »
Translated abstract:: Isoformen 1A1 und 1A3 des Enzyms Aldehyd Dehydrogenase 1 co-lokalisieren in humanen Satellitenzellen, den Stammzellen der Skelettmuskulatur. Die Funktion von 1A1 und 1A3 in Myogenese soll untersucht werden. Hier konnte ich zeigen, dass 1A1 und 1A3 Proteine essenziell für die Muskeldifferenzierung sind, da ein Isoform Knockout zu einem Verlust des Differenzierungspotentials führt. Re-epxression in Knockout Zellen stellt Differenzierung wieder her. Chemische ALDH Inhibition führt zu einer Akkumulation der Isoform Proteine und resultiert in Differenzierungsverhalten. «
Isoformen 1A1 und 1A3 des Enzyms Aldehyd Dehydrogenase 1 co-lokalisieren in humanen Satellitenzellen, den Stammzellen der Skelettmuskulatur. Die Funktion von 1A1 und 1A3 in Myogenese soll untersucht werden. Hier konnte ich zeigen, dass 1A1 und 1A3 Proteine essenziell für die Muskeldifferenzierung sind, da ein Isoform Knockout zu einem Verlust des Differenzierungspotentials führt. Re-epxression in Knockout Zellen stellt Differenzierung wieder her. Chemische ALDH Inhibition führt zu einer Akkumula... »
WWW:: https://mediatum.ub.tum.de/?id=1553081
Date of submission:: 09.07.2020
Oral examination:: 11.11.2020
File size:: 3876850 bytes
Pages:: 98
Urn (citeable URL):: https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20201111-1553081-1-7
Last change:: 25.11.2020
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