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Titel:

Impact of Nanoparticle Aggregation on Protein Recovery through a Pentadentate Chelate Ligand on Magnetic Carriers

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Fraga García, P.; Freiherr von Roman, M.; Reinlein, S.; Wolf, M.; Berensmeier, S.
Abstract:
The growing need for more efficient separation techniques still dominates downstream processing of biomolecules, thus encouraging the continuous development of advanced anomaterials. In this paper we present an improved process for recovering recombinant histidine tagged green fluorescent protein from an E. coli cell lysate. Superparamagnetic ore−shell nanocarriers are functionalized with a pentadentate chelate affinity ligand and then loaded with metal ions (Cu2+, Ni2+, or Zn2+). The separatio...     »
Stichworte:
protein recovery; biomolecule purification; magnetic separation; pentadentate chelate ligand; aggregation; agglomeration; nanoparticles
Zeitschriftentitel:
ACS Applied Materials & Interfaces
Jahr:
2014
Jahr / Monat:
2014-06
Sprache:
en
Volltext / DOI:
doi:10.1021/am503082s
WWW:
http://pubs.acs.org/doi/abs/10.1021/am503082s
TUM Einrichtung:
Fachgebiet für Selektive Trenntechnik
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