In this thesis the development of a quasi-continuous combined system is described, which allows a fast and selective enrichment of bacteria in drinking water. The system can be combined with bioanalytical methods and offers the possibility to enrich bacterial cells from a large volume (10 L) to a small volume (200 µL) within 30 minutes. Two different separation techniques were combined in the system: the cross-flow microfiltration (CFM) and the affinity chromatography. In the first step, the microorganisms are concentrated by size-exclusion automatically with the CFM to a volume of 50 mL. This rapid enrichment process enabled recovery rates of 903% of the bacteria cells. In the second step, the bacterial cells are separated and concentrated (up to a volume of 200 µL) with a monolithic affinity column. The separation takes place under physiological conditions and allows a complete enrichment of the cells (983%).
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In this thesis the development of a quasi-continuous combined system is described, which allows a fast and selective enrichment of bacteria in drinking water. The system can be combined with bioanalytical methods and offers the possibility to enrich bacterial cells from a large volume (10 L) to a small volume (200 µL) within 30 minutes. Two different separation techniques were combined in the system: the cross-flow microfiltration (CFM) and the affinity chromatography. In the first step, the mic...
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