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Title:

Hot Flows: Evolving an Archaeal Glucose Dehydrogenase for Ultrastable Carba-NADP+ Using Microfluidics at Elevated Temperatures

Document type:
Zeitschriftenaufsatz
Author(s):
Zachos, Ioannis; Genth, Robert; Sutiono, Samuel; Marczynski, Matthias; Lieleg, Oliver; Sieber, Volker
Abstract:
Here, we describe the successful engineering of a promiscuous glucose dehydrogenase from the thermophilic archaeon, Saccharolobus solfataricus, toward the thermostable and robust cofactor, carba-NADP+, using a photometric-based microfluidic screening system at elevated temperatures. We show that droplets encapsulating single enzyme variants with the right handling could sufficiently sustain long incubation time at elevated temperatures. Furthermore, we enabled an ultrahigh-throughput screening f...     »
Keywords:
artificial nicotinamide biomimetic; cofactors carba-NADP+; cofactor recycling; enzyme engineering; microfluidics; droplet screening system
Dewey Decimal Classification:
500 Naturwissenschaften
Journal title:
ACS Catalysis
Year:
2022
Journal volume:
12
Journal issue:
3
Pages contribution:
1841-1846
Covered by:
Scopus
Reviewed:
ja
Language:
en
Fulltext / DOI:
doi:10.1021/acscatal.1c04320
WWW:
https://pubs.acs.org/doi/abs/10.1021/acscatal.1c04320
Publisher:
American Chemical Society (ACS)
E-ISSN:
2155-54352155-5435
Date of publication:
19.01.2022
Semester:
WS 21-22
TUM Institution:
Fachgebiet für Biomechanik
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