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Title:

Fluorophore-conjugated Helicobacter pylori recombinant membrane protein (HopQ) labels primary colon cancer and metastases in orthotopic mouse models by binding CEA-related cell adhesion molecules.

Document type:
Article; Journal Article
Author(s):
Hollandsworth, Hannah M; Schmitt, Verena; Amirfakhri, Siamak; Filemoni, Filemoni; Schmidt, Alexej; Landström, Maréne; Lyndin, Mykola; Backert, Steffen; Gerhard, Markus; Wennemuth, Gunther; Hoffman, Robert M; Singer, Bernhard B; Bouvet, Michael
Abstract:
HopQ is an outer-membrane protein of Helicobacter pylori that binds to human carcinoembryonic antigen-related cell-adhesion molecules (CEACAMs) with high specificity. We aimed to investigate fluorescence targeting of CEACAM-expressing colorectal tumors in patient-derived orthotopic xenograft (PDOX) models with fluorescently labeled recombinant HopQ (rHopQ). Western blotting, flow cytometry and ELISA were performed to determine the efficiency of rHopQ binding to CEACAMs. rHopQ was conjugated to I...     »
Journal title abbreviation:
Transl Oncol
Year:
2020
Journal volume:
13
Journal issue:
12
Fulltext / DOI:
doi:10.1016/j.tranon.2020.100857
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/32866936
Print-ISSN:
1936-5233
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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