Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Polyethylene terephthalate (PET) is responsible for a large part of environmental contamination with microplastics. Based on the high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET’s crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.     «

Polyethylene terephthalate (PET) is responsible for a large part of environmental contamination with microplastics. Based on the high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET’s crystallinity. The separation or decomposition of...     »