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Titel:

Magnetic separation of antibodies with high binding capacity by site-directed immobilization of Protein A-domains to bare iron oxide nanoparticles

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Kaveh-Baghbaderani Y, Allgayer R, Schwaminger SP, Fraga-Garca P, Berensmeier S:
Abstract:
The demand for purified antibodies is ever-rising. This study presents a nanoparticle-based material for efficient magnetic separation of immunoglobulin G (IgG) with high binding capacity. The characteristics include: (i) Cost-effective bare iron oxide nanoparticles are used as the solid phase on which optimized protein A-based ligands are directly immobilized. An additional chemical modification or activation is not needed. (ii) Oriented immobilization of the ligands is promoted using a C-termi...     »
Stichworte:
affinity tag antibody purification iron oxide nanoparticles magnetic separation protein A
Zeitschriftentitel:
ACS Applied Nano Materials
Jahr:
2021
Sprache:
en
WWW:
https://pubs.acs.org/doi/10.1021/acsanm.1c00487
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