Benutzer: Gast  Login
Titel:

High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Dölken, L; Ruzsics, Z; Rädle, B; Friedel, CC; Zimmer, R; Mages, J; Hoffmann, R; Dickinson, P; Forster, T; Ghazal, P; Koszinowski, UH
Abstract:
RNA levels in a cell are determined by the relative rates of RNA synthesis and decay. State-of-the-art transcriptional analyses only employ total cellular RNA. Therefore, changes in RNA levels cannot be attributed to RNA synthesis or decay, and temporal resolution is poor. Recently, it was reported that newly transcribed RNA can be biosynthetically labeled for 1-2 h using thiolated nucleosides, purified from total cellular RNA and subjected to microarray analysis. However, in order to study sign...     »
Zeitschriftentitel:
RNA
Jahr:
2008
Band / Volume:
14
Heft / Issue:
9
Seitenangaben Beitrag:
1959-72
Sprache:
eng
Volltext / DOI:
doi:10.1261/rna.1136108
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/18658122
Print-ISSN:
1355-8382
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
 BibTeX