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Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article 
Author(s):
Dorsch, S; Klotz, KN; Engelhardt, S; Lohse, MJ; Bünemann, M 
Title:
Analysis of receptor oligomerization by FRAP microscopy. 
Abstract:
Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP. We validated this approach with CD86 and CD28 as monomeric and dimeric reference proteins, respectively. Di- or oligomerization of G protein-coupled receptors is strongly debated. We studied human...    »
 
Journal title abbreviation:
Nat Methods 
Year:
2009 
Journal volume:
Journal issue:
Pages contribution:
225-30 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
1548-7091 
TUM Institution:
Institut für Pharmakologie und Toxikologie 
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