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Title:

Selective Ene-Reductase Immobilization to Magnetic Nanoparticles through a Novel Affinity Tag

Document type:
Zeitschriftenaufsatz
Author(s):
Zanker A., Ahmad N., Hoang Son T., Schwaminger S., Berensmeier S.:
Abstract:
Background: Magnetic nanoparticles (MNPs) are becoming more important as carriers, because of their large specific surface area and easy separability. Magnetic nanoparticles are increasingly used in enzyme technology, diagnostics, and drug delivery. Major results: For the directed and almost irreversible immobilization of proteins on MNPs, we have developed a new selective (His-Arg)4 peptide-tag, that binds fusion proteins directly from an E. coli cell lysate to non-functionalized, low-cost...     »
Keywords:
affinity tag, ene-reductase, enzyme immobilization, iron oxides, magnetic nanoparticles
Journal title:
Biotechnology Journal
Year:
2021
Year / month:
2021-04
Covered by:
Scopus
Reviewed:
ja
Language:
en
Fulltext / DOI:
doi:10.1002/biot.202000366
WWW:
https://doi.org/10.1002/biot.202000366
Publisher:
Wiley
Impact Factor:
3.91
Submitted:
26.08.2020
Accepted:
10.11.2020
Date of publication:
27.11.2020
TUM Institution:
Professur für Selektive Trenntechnik
Ingested:
01.12.2020
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