We developed a short-term feeder cell-free single CD8+ T cell expansion protocol that allows validation of pMHC multimer reactivity as well as structural TCR avidity measurement before TCR identification. In this way, we identified 28 TCRs which were all transgenically re-expressed. Additionally, we developed a novel double-tagged pMHC monomer which allows versatile probe conjugation to generate distinct pMHC reagents from a single precursor. This new pMHC reagents could be combined with the di-peptide exchange technique for rapid peptide exchange.
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We developed a short-term feeder cell-free single CD8+ T cell expansion protocol that allows validation of pMHC multimer reactivity as well as structural TCR avidity measurement before TCR identification. In this way, we identified 28 TCRs which were all transgenically re-expressed. Additionally, we developed a novel double-tagged pMHC monomer which allows versatile probe conjugation to generate distinct pMHC reagents from a single precursor. This new pMHC reagents could be combined with the di-...
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