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Title:

Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Franken, Lars; Klein, Marika; Spasova, Marina; Elsukova, Anna; Wiedwald, Ulf; Welz, Meike; Knolle, Percy; Farle, Michael; Limmer, Andreas; Kurts, Christian
Abstract:
A main function of splenic red pulp macrophages is the degradation of damaged or aged erythrocytes. Here we show that these macrophages accumulate ferrimagnetic iron oxides that render them intrinsically superparamagnetic. Consequently, these cells routinely contaminate splenic cell isolates obtained with the use of MCS, a technique that has been widely used in immunological research for decades. These contaminations can profoundly alter experimental results. In mice deficient for the transcript...     »
Journal title abbreviation:
Sci Rep
Year:
2015
Journal volume:
5
Pages contribution:
12940
Language:
eng
Fulltext / DOI:
doi:10.1038/srep12940
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/26260698
Print-ISSN:
2045-2322
TUM Institution:
Roman Herzog Comprehensive Cancer Center
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