User: Guest  Login
Title:

Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones

Author(s):
Laner, A.; Schwarz, T.; Christan, S.; Schindelhauer, D.
Abstract:
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (BS) selection, a CMV/EGFP expression cassette was inserted into a HAC construct based on chromosome 5 alpha satellite DNA (142 kb). Lipofection into HT1080 cells resulted in a small proportion of cells exhibiting bright green fluorescence on day...     »
Journal title:
Cytogenetic and Genome Research
Year:
2004
Journal volume:
107
Journal issue:
1-2
Pages contribution:
9--13
Fulltext / DOI:
doi:10.1159/000079564
Publisher:
S. Karger AG
Publisher address:
Basel, Switzerland
Print-ISSN:
1424-859X
E-ISSN:
1424-859X
Notes:
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.
 BibTeX