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Titel:

Detection and differentiation of murine leukemia virus (MLV) and murine stem cell virus (MSCV) and therefrom derived nucleic acids.

Dokumenttyp:
Article; Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Volkwein, Wolfram; Pavlovic, Melanie; Anton, Martina; Haase, Maren; Stellberger, Thorsten; Jarrar, Amin; Busch, Ulrich; Baiker, Armin
Abstract:
Murine leukemia virus (MLV) and murine stem cell virus (MSCV) and derived retroviral vectors are widely used to study retrovirus biology and as tools for gene delivery. The method described here represents a quantitative real time PCR (qPCR) with hydrolysis probe that can be applied within classical qPCR as well as in digital droplet PCR (ddPCR). The method targets a 60 bp long fragment located within the U5 region of the MLV/MSCV genome sequence. For the here described method a LOD95% of 25 cop...     »
Zeitschriftentitel:
J Virol Methods
Jahr:
2022
Band / Volume:
299
Volltext / DOI:
doi:10.1016/j.jviromet.2021.114316
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/34627947
Print-ISSN:
0166-0934
TUM Einrichtung:
Roman Herzog Comprehensive Cancer Center
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