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Titel:

Expression, one-step purification, and immobilization of HaloTag(TM) fusion proteins on chloroalkane-functionalized magnetic beads

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Motejadded H; Kranz B; Berensmeier S; Franzreb M; Altenbuchner J.
Abstract:
The presented work introduces a novel method to immobilize enzymes either purified or directly out of a crude extract onto magnetic particles in the micrometer range. This method is based on the creation of a fusion protein consisting of the enzyme of choice and a mutant dehalogenase. The dehalogenase gene is commercially available from the company Promega under the name HaloTag(TM). When the fusion protein is contacted with magnetic beads having chemically synthesized, chloroalkane ligands on t...     »
Zeitschriftentitel:
Appl Biochem Biotechnol.
Jahr:
2010
Jahr / Monat:
2010-11
Sprache:
en
Volltext / DOI:
doi:10.1007/s12010-010-8985-1
WWW:
http://www.ncbi.nlm.nih.gov/pubmed/20473582
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