Microbial communities, consisting, for instance, of bacteria, archaea, and yeasts are often determined using sequencing of the 16S and 18S rRNA, respectively, and subsequent comparison with databases. Here, different methods producing short, long, or synthetic full-length sequences were either enhanced or newly established, tested, and compared, using, e.g., artificial mock communities, human stool, and cow milk. The developments made allow improved accuracy of the classification, estimation of the comparability of studies, and using samples with low amounts of organisms.
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Microbial communities, consisting, for instance, of bacteria, archaea, and yeasts are often determined using sequencing of the 16S and 18S rRNA, respectively, and subsequent comparison with databases. Here, different methods producing short, long, or synthetic full-length sequences were either enhanced or newly established, tested, and compared, using, e.g., artificial mock communities, human stool, and cow milk. The developments made allow improved accuracy of the classification, estimation of...
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